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A Gel-Delivery System for Coccidiosis Vaccine:
Uniformity
of Distribution of Oocysts
Tapashi
Dasgupta, Eng Hong Lee
(In
Print: Canadian Veterinary Journal)
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A
representative CDC anaerobic blood agar plate. The figure
indicates a uniform
distribution of the organisms over the entire
sausage slice
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Abstract
A
patented gel delivery system being used to deliver coccidiosis vaccine
to poultry hatchlings is assessed. For effective
vaccination, the coccidial oocysts must be uniformly suspended before
exposure to birds. The uniformity of distribution within the
gel was evaluated by incorporating a culture of chicken gut flora into
four replicate gel sausages, placing sections of the sausages on
culture plates, determining the appearance and distribution of
bacterial colonies on culture plates after incubation under aerobic and
anerobic conditions, and verifying by cell counts (Table 1). The
uniformity of distribution of similarly prepared coccidial
oocysts was verified by infecting birds with 40,000 E.
tenella oocysts delivered via the gel.
Gel-inoculated birds were compared with control birds inoculated per
os with 40,000 oocysts suspended
in
water by
using cecal lesion scores (Table 2). Both the
appearance and colony counts of chicken gut flora from the gel were
uniform. The standard deviation in the lesion scores for the
gel-inoculated group and the water-inoculated groups was 0.51 and 0.69,
respectively. The results indicate that a gel-delivery system
can provide uniform distribution of live organisms and vaccine agents
to birds.
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Table 1: Colony Forming Units Obtained on CDC anaerobic blood
agar
plates:
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Replicate
#1 |
Replicate
#2 |
Replicate
#3 |
Replicate
#4 |
|
Aer. |
Anaer. |
Aer. |
Anaer. |
Aer. |
Anaer. |
Aer. |
Anaer. |
| TOP |
350 |
410 |
358 |
428 |
359 |
420 |
362 |
400 |
| MIDDLE |
365 |
412 |
362 |
425 |
365 |
435 |
368 |
435 |
| BOTTOM |
380 |
420 |
365 |
430 |
380 |
435 |
379 |
420 |
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Table
2:Summary
of lesion scores in infected birds:
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Mean
lesion
score
|
Standard
deviation (S) |
P-value |
| Gel-inoculated
group |
+3.1
|
0.51
|
<
0.01 |
| Water-inoculated
group |
+2.87
|
0.69
|
<
0.01 |
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Disclaimer
Copyright © 2007, Vetech Laboratories Inc.
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